22. European Stroke Conference 8 Experimental studies A 15:40 - 15:50 In vitro examination of the thrombolytic efficiency of Desmoteplase compared with rt-PA and the additional application of ultrasound Z. Wang1, F. Roessler2, S. Schumacher3, M. Ohlrich4, A. Menciassi5, J. Eggers6 The BioRobotics Institute, Scuola Superiore Sant’Anna and Center for Micro-BioRobot-ics@ SSSA, Istituto Italiano di Tecnologia, Pisa, ITALY1,Department of Neurology, Uni-versity Hospital Schleswig-Holstein, Campus Lübeck, Luebeck, GERMANY2, Department of Neurology, University Hospital Schleswig-Holstein, Campus Lübeck, Luebeck, GERMA-NY3, Department of Neurology, University Hospital Schleswig-Holstein, Campus Lübeck, Luebeck, GERMANY4, The BioRobotics Institute, Scuola Superiore Sant’Anna and Center for Micro-BioRobotics@SSSA, Pisa, ITALY5, Department of Neurology, University Hospital Schleswig-Holstein, Campus Lübeck, Luebeck, GERMANY6 Background: Desmoteplase (DSPA) is a novel fibrin-selective plasminogen activator which is a promising candidate for the thrombolytic treatment of acute ischemic stroke. The purpose of this study was to determine the thrombolytic efficiency of DSPA compared with rt-PA and the additional effect of ultrasound (US) application in vitro. Methods: A static model suitable for the application of thrombolytic drugs and US was built. Dose finding experiments of DSPA were made with concentrations of 0.1μg/ml up to 10μg/ ml. Lysis rate of clots made from platelet rich plasma (PRP) was measured by means of clots weight loss after one hour for 5 different groups (each n= 40): In the control group clots were solely treated with plasma (pH=7.4) and in the other 4 groups with a solution of plasma and rt- PA ( 60 kU/ml) or plasma and DSPA (2 μg/ml) each with or without diagnostic ultrasound (2 MHz, 0.179 W/cm2). Results: Clot lysis rate showed saturation above DSPA concentrations of 2μg/ml in one hour. A 2:2 factorial ANOVA showed significant main effects for both factors MEDICATION and US concerning lysis rate without significant interaction. Post hoc T-tests revealed significant increase of clot lysis with rt-PA (27 ± 8 %) and DSPA (31 ± 7 %) compared with control (18 ± 6 %, each p<0.001). The additional application of US led to a no significant increased lysis for rt-PA (29 ± 6 %) but a significant increased lysis for DSPA (33 ± 7 %, p=0.049). Lysis by DSPA was more effective compared with rt-PA (without US: p=0.03; with US: p=0.009). Conclusions: Our data indicated that the thrombolytic efficiency of DSPA is significantly higher compared with rt-PA both with or without US. 60 © 2013 S. Karger AG, Basel Scientific Programme
Karger_ESC London_2013
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