22. European Stroke Conference 544 © 2013 S. Karger AG, Basel Scientific Programme 483 Experimental studies EFFECTS OF LITHIUM AND LAMOTRIGINE ON OXIDATIVE STRESS AND SPATIAL LEARNING DEFICIT AFTER GLOBAL CEREBRAL ISCHEMIA A. OZKUL1, A. SAIR2, A. AKYOL3, C. YENISEY4, T. DOST5, C. TATAROGLU6 Adnan Menderes University, Faculty of Medicine, Department of Neurology, Aydin, Turkey, AYDIN, TURKEY1, Adnan Menderes University, Faculty of Medicine, Department of Neurology, Aydin, Turkey, AYDIN, TURKEY2, Adnan Menderes University, Faculty of Medicine, Department of Neurology, Aydin, Turkey, AYDIN, TURKEY3, Adnan Menderes University, Faculty of Medi-cine, Department of Biochemistry, Aydin, Turkey, Aydin, TURKEY4, Adnan Menderes University, Faculty of Medicine, Department of Pharmacology, Aydin, Turkey, Aydin, TURKEY5, Adnan Men-deres University, Faculty of Medicine, Department of Pathology, Aydin, Turkey, Aydin, TURKEY6 Background: Lithium and lamotrigine are two major drugs having neuroprotective properties. How-ever, the exact therapeutic mechanisms of these drugs have not been well understood. Methods: In this study we investigated the antioxidant properties of lithium (40mg/kg/day and 80mg/kg/day) and lamotrigine (20mg/kg/day and 40mg/kg/day) in a rat model of global cerebral ischemia based on permanent bilateral occlusion of the common carotid arteries (BCAO). Nitric oxide (NO), cata-lase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), GSH (glutathione) and GSH-R (glutathione reductase) levels were measured as an indicator of oxidative stress in both cortex and hippocampal tissue after 28 days of treatment. The ischemia-induced spatial and learning disability was also assessed by Morris water maze (MWM) test. Results: All studied oxidative stress markers were found to be higher in the BCAO group than in sham operated rats. Both Li and LTG treatments caused a decrease in NO and MDA elevation, meanwhile the increase in GSH, GSH-R, CAT and SOD levels was significantly more evident in treated groups than in the BCAO group. Although all studied oxidative parameters showed significant differences between groups in cortical tissue, the similiar differences could be detected only in MDA, CAT and SOD in analyses of hippocampal sam-ples. We also found higher cortical GSH-R and hippocampal SOD levels in BCAO+ Li (80mg/d) treated group when compared with BCAO+LTG 40mg/d. MWM test data showed a similiar increase in spatial and learning ability in all groups under treatment. We found no other statistically signifi-cancy in comparison of treated groups with different dosages. Conclusion: Our findings suggested that Li and LTG treatments may inhibit spatial and learning memory deficits accompanied by lower oxidative stress in global cerebral ischemia. Formulating the mechanism of Li and LTG may have potential benefits for the treatment of vascular dementia in clinical practice.
Karger_ESC London_2013
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