London, United Kingdom 2013 Poster Session Red Cerebrovasc Dis 2013; 35 (suppl 3)1-854 543 481 Experimental studies Effect of Combination of Edible Oils on Blood Pressure, Lipid Profile, Lipid Peroxidative Markers, Antioxidant Status, and Electrolytes in Patients with Hypertension on Nifedipine Treatment G. Chandramohan1, K.S. Al-Numair2, M.A. Alsaif3, B. Sudhakar4, K.V. Pugalendi5 Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, SAUDI ARABIA1, Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, SAUDI ARABIA2, Department of Com-munity Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, SAUDI ARABIA3, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamilnadu, India, Chidambaram, INDIA4, Department of Bio-chemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamilnadu, India, Chidambaram, INDIA5 Background: To determine the effect of combination of edible oils on blood pressure, anthropomet-ric parameters, lipid profile, lipid peroxidative markers, antioxidant status and electrolytes in drug (nifedipine) taking patients with hypertension. Methods: In this study, patients were separated into 4 groups. Normal (n=14), hypertensive patients (n=38), 38 patients under medication with nifedipine were divided into 2 groups nifedipine control (n=12) and nifedipine + oil combination (sesame + sunflower oil) groups (n=26). Sesame and sun-flower oil combination was supplied to patients and instructed to use it as the only oil source for 45 days. Blood pressure and anthropometric parameters were measured at baseline and after 45 days. Lipid peroxidative markers, enzymatic and non-enzymatic antioxidants, lipid profile and electrolytes in blood were also measured. The study took place at Rajah Muthiah Medical College and Hospital, Annamalai University, Annamalainagar, India. Results: Nifedipine and oil-mix consumed patients significantly decreased the blood pres-sure, lipid peroxidative markers, lipid profile excluding the high density lipoprotein cholesterol (HDL-C), sodium, chloride, and increased enzymatic, non-enzymatic antioxidants, HDL-C and potassium levels when compared to nifedipine alone treated hypertensive patients. Conclusion: Nifedipine and oil-mix provided good protection over blood pressure and lipid per-oxidation, and brought enzymatic and non-enzymatic antioxidants, lipid profile, and electrolytes towards normalcy in hypertensive patients. 482 Experimental studies Neuroprotective effect of herbal extract containing Gastrodiae Rhizoma and Uncariae Ramu-lus cum Uncis on middle cerebral artery occlusion in rat J.W. XIAN1, C.W. CHAN2, BS LAU3, C.M. KOON4, C.H. KO5, W.N. LEUNG6 The Chinese University of Hong Kong, Hong Kong, HONG-KONG1, The Chinese University of Hong Kong, Hong Kong, HONG-KONG2, The Chinese University of Hong Kong, Hong Kong, HONG-KONG3, The Chinese University of Hong Kong, Hong Kong, HONG-KONG4, The Chinese University of Hong Kong, Hong Kong, HONG-KONG5, The Chinese University of Hong Kong, Hong Kong, HONG-KONG6 Background: Stroke is the second major cause of death in the world. Tissue plasminogen activator (t-PA) is the only approved therapy for acute ischemic stroke (NINDS, 1995). However, t-PA is re-quired given to patient within 3 hours post-stroke. Such a short period of t-PA treatment require al-ternative therapy for cerebral ischemia. Water extract of Gastrodiae Rhizoma (TM) and Uncariae Ramulus cum Uncis (GT) have been used to treat as anticonvulsant, analgesic, antipyretic and antihypertensive drug and cerebral disorder for hundred years in China. The study aims to investigate the treatment effect of a Traditional Chinese medicine formula which composes of TM and GT (TM: GT= 3:4, w/w, TGW) on middle cerebral artery occlusion in rat. Method: After SD rats were subject to surgery of middle cerebral artery occlusion (MCAO), they were given TGW (low, middle, high dose) or PBS (vehicle of TGW) by oral gavage daily. Neurolog-ical deficit score examination and pole test were performed 24h after MCAO and daily throughout 7-day experiment. Rat brain was harvested at 7-day post-operatively and sectioned in 2mm thick. Brain slice is stained with 2% TTC to evaluate cerebral infarction volume. Results: After 7-day treatment of TGW, the ratio of infract volume decreased from 45.6% (Fig. A, control) to 30.3% (low dose), 23.8% (mid dose) and 14.1% (high dose) (Fig. B-D). The neurological deficit score of MCAO reduced from 2.86 to 0.80 (low dose), 0.55 (mid dose) and 0.50 (high dose) (Fig. F). TGW administered induced neurological deficit score and infract volume in a dose-depen-dent manner. Furthermore, pole test of TGW treated rat retained result (E) took shorter time to walk through the pole and was comparable to sham group at day 3. Conclusion: Water extract of Gastrodiae Rhizoma and Uncariae Ramulus cum Uncis might have protective effect against cerebral ischemia/reperfusion of middle cerebral artery occlusion in rat. The mechanism of neuroprotective effect of TGW will be studied.
Karger_ESC London_2013
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