London, United Kingdom 2013 Poster Session Red Cerebrovasc Dis 2013; 35 (suppl 3)1-854 539 474 Experimental studies The effects of G-CSF and bone marrow-derived mononuclear cells after photothrombotic stroke in spontaneously hypertensive rats. A. Schmidt1, J.K. Strecker2, C. Beuker3, W.R. Schäbitz4, J. Minnerup5, K. Diederich6 Department of Neurology, University of Münster, Münster, GERMANY1, Department of Neu-rology, University of Münster, Münster, GERMANY2, Department of Neurology, University of Münster, Münster, GERMANY3, Department of Neurology, EVK Bielefeld, Bethel, Bielefeld, GER-MANY4, Department of Neurology, University of Münster, Münster, GERMANY5, Department of Neurology, University of Münster, Münster, GERMANY6 Introduction: In animal models of stroke, granulocyte-colony stimulating factor (G-CSF) is neuro-protective and promotes angiogenesis and neurogenesis, partly by mobilising haematopoietic stem cells. Apart from that, therapies with exogenous bone marrow-derived mononuclear cells (BM-MNCs) have also shown beneficial effects in animal models of stroke. We compared the efficacy of G-CSF, BM-MNCs and a combination therapy of G-CSF and BM-MNCs after photothrombotic stroke in spontaneously hypertensive rats Methods: Photothrombotic cortical infarcts were induced in 60 spontaneously hypertensive adult male rats. Sixty minutes after photothrombosis, animals received either a single injection of 5 mil-lion pkh26-labelled BM-MNCs intravenously (n=15), or a single injection of 5 million BM-MNCs intravenously plus 12.5 million IE G-CSF/d for five days i.p. (n =15), or 12.5 million IE G-CSF/d for five days i.p. (n=15), or placebo (n=15). The adhesive tape removal test was performed to assess the functional outcome. Currently, histological analyses are performed to analyse the effects on en-dogenous repair mechanisms. Results: Four weeks after photothrombotic ischemia, the adhesive tape removal test demonstrated that the monotherapy with G-CSF significantly improved the functional recovery compared to pla-cebo (p = 0.001), and compared to the monotherapy with BM-MNCs (p < 0.05). BM-MNCs alone and the combination therapy of G-CSF and BM-MNCs did not significantly improve the functional outcome. Conclusion: Our results confirm that G-CSF improves the functional recovery in animal models of stroke. G-CSF achieved better results than BM-MNCs and a combination of G-CSF and BM-MNCs. 475 Experimental studies The dopamine treatment improved the functional recovery and modulated the inflammatory reactions at acute cerebral infarction on photochemical ischemic animal model B.S. SHIN1, Z. JIN2 Department of Neurology, Chonbuk National University Medical School, Chonbuk National University Hospital Research Institute of Clinical Medicine, Jeonju, SOUTH KOREA1, De-partment of Medical Science, Chonbuk National University Graduate School, Jeonju, SOUTH KO-REA2 Backgrounds: Cerebral ischemic injury triggers inflammatory responses that play a critical role in disease progression, cellular reorganization and functional recovery. Dopamine is a catecholamine neurotransmitter that modulates neuronal activity in several brain regions. Several studies show that dopamine (DA) treatment contribute to recovery of sensorimotor function and to procedural motor learning, and modulate neuronal activity in several brain regions and functional networks. Further-more, dopamine modulate immune functions through D1 and D2 classes of receptors present in the target cells, which exerts a variety of physiological effects in the healthy or diseased brain. The purpose of this study is to evaluate the effect of dopaminergic treatment in acute ischemic stroke for functional recovery and its correlation with modulation of inflammatory reaction. Methods: The cortical photothrombosis were induced on the unilateral sensorimotor cortex in Sprague-Dawley rats. The dopamine group were treated for 5days after ischemia injury (10mg/kg, 20mg/kg, 50mg/ kg, and 100mg/kg) and compared with saline group. The behavior function was evaluated with beam walking test and cylinder test. The immunohistochemical studies used for measurement of in-farction volume, apoptosis and neuroprotection. We measured expression of DR1, DR2, Bax, Bcl-2, IL-6, NGF in cortical area. Results: The motor function was improved in dopamine treatment groups during five consecutive days (p<0.05). Furthermore, the cortical infarct volume and apoptotic cell death was decreased and neuroprotection was increased in dopamine treatment group. In the west-ern blot, the expression of DR1, DR2, Bc1-2 was increased (p<0.01), and the expression of Bax and IL-6 was decreased by dopamine receptors expression (p<0.01). Conclusion: The early dopamine treatment induced the functional recovery and decreased the brain injury after acute ischemic stroke. The mechanism of neuroprotective effect of dopamine is correlated with expression of dopamine re-ceptors and anti-inflammatory reaction. We support the evidence of clinical trial with dopamine for functional recovery in acute cerebral infarction patients.
Karger_ESC London_2013
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