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22. European Stroke Conference 267 Etiology of stroke and risk factors Epidemiological, genetic features of personal anxiety as a risk factor for stroke (WHO “MONICA-psychosocial”) V.V. Gafarov1, E.A. Gromova2, M.I. Voevoda3, V. Maksimov4, N. Judin5, T. Mishakova6, I.V. Gagulin7, A.V. Gafarova8 Collaborative laboratory of cardiovascular diseases epidemiology SB RAMS, Novosibirsk, RUSSIAN FEDERATION1, Institute of Internal MedicineSB RAMS, Novosibirsk, RUSSIAN FEDERATION2, Institute of Internal MedicineSB RAMS, Novosibirsk, RUSSIAN FEDERATION3, Institute of Internal MedicineSB RAMS, Novosibirsk, RUSSIAN FEDERATION4, Institute of Cy-tology and Genetic SB RAS, Novosibirsk, RUSSIAN FEDERATION5, Institute of Cytology and Genetic SB RAS, Novosibirsk, RUSSIAN FEDERATION6, Collaborative laboratory of cardiovas-cular diseases epidemiology SB RAMS, Novosibirsk, RUSSIAN FEDERATION7, Collaborative laboratory of cardiovascular diseases epidemiology SB RAMS, Novosibirsk, RUSSIAN FEDERA-TION8 Background: Study the association of high level of anxiety (HLA) with VNTR polymorphisms D4 and DAT genes; determine risk of stroke at men with HLA. Methods: Within the framework of program WHO MONICA-MOPSY a representative sample of men 25-64 years old (1984, 1988, 1994 years) was examined. The total sample was 2149 persons. The period of supervision was 24 years. We counted as outcome all cases of stroke which had arisen for the first time. We used Spielberger’s scale for estimation of personal anxiety (PA). Cox-propor-tional regression model was used for estimation of hazard ratio (HR). Results: 72.2% men with developed stroke had HLA and 27.8% ALA (average level of anxiety). Risk of developed stroke within 5 years in a group of men with HLA in comparison with a group of men with ALA was 6.4 (95%CI 3-13.3, p<0.001) times, 10 years HR =3.8 (95%CI 1.6-8.7, p<0.001), 15 years HR= 2.9 (1.1-7.4, p<0.05) times higher. Within 20 and 24 years the tendency to RR decrease for development (HR=1.6 and HR=1.04) was observed. Since HLA genotype was sig-nificantly associated 4/6 DRD4 gene and genotype 9/9 gene DAT. Conclusion: There is high prevalence of HLA at male aged 25-64 in Russian. HLA were significant-ly associated with certain VNTR polymorphisms of genes DRD4, DAT; HLA increases the stroke in the first five years. 428 © 2013 S. Karger AG, Basel Scientific Programme 268 Etiology of stroke and risk factors Methylenetetrahydrofolate Reductase Polymorphisms C677T and A1298C as Important Ge-netic Risk Factors in Pediatric Stroke Pathway A. KOLK1, R. Laugesaar2, T. Kahre3, E.-M. Lotman4, T. Nikopensius5, T. Talvik6 University of Tartu, Tartu University Hospital, Tartu, ESTONIA1, University of Tartu, Tartu University Hospital, Tartu, ESTONIA2, University of Tartu, Tartu, ESTONIA3, University of Tartu, Tartu, ESTONIA4, Biobank of the Estonian Genome Center, Tartu, ESTONIA5, University of Tartu, Tartu University Hospital, Tartu, ESTONIA6 Background: Pediatric stroke is increasingly gaining clinical attention; among its risk factors, meth-ylenetetrahydrofolate reductase (MTHFR) impaired activity depending on common mutations (677C>T and 1298A>C) is considered. Our aim was to explore the role of the two MTHFR poly-morphisms in Estonian pediatric stroke population, to compare it with population-based data and to examine possible genotype differences between pediatric stroke subtypes. Methods: There were 59 patients with pediatric stroke (37 boys and 22 girls) selected out of 144 patients from the Estonian Pediatric Stroke Registry (during the period from 2004 to 2012). There were 51 children with arterial ischemic stroke (AIS) and 8 with hemorrhagic stroke (HS). Control group consisted of 763 (388 males and 375 female) randomly selected healthy young persons (Bio-bank of the Estonian Genome Center). MTHFR polymorphisms were genotyped using PCR-RFLP analysis. Results: The 677TT and the 1298CC genotypes showed a frequency of 10.9% (95% C.I. 2.7 – 19.1) and 6.8% (95% C.I. 0.4 – 13.2) respectively in stroke patients. The 677TT and the 1298CC geno-type frequencies in controls were 7% and 6% respectively. In children with stroke the frequencies of the variant alleles were 49.2% for 677T and 49.2% for 1298C. In the AIS group allele frequencies were 49% and 43.1% and in the HS group 50% and 87.5% respectively. Conclusion: A higher prevalence of MTHFR polymorphism was found in stroke group. Wild gen-otype in stroke group was found only in 18% of children. Hemorrhagic stroke group demonstrated the highest prevalence of the 1298AC/CC genotype - in 87.5% of cases. Our data do support the hypothesis that the two MTHFR polymorphisms may represent an important genetic risk factor for pediatric stroke. Furthermore, the frequency of the MTHFR polymorphisms could induce consider-ation of a nutrition policy based on folic acid supplementation.


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