252 Scientific Programme 22. European Stroke Conference © 2013 S. Karger AG, Basel 10 Experimental studies Evaluation of the safety and efficacy of the therapeutic potential of adipose tissue stem cells in cerebral ischemic penumbra U. Gómez-Pinedo1, J.A. Matias-Guiu2, G. León-Espinoza3, C. Lendinez4, M.S. Benito5, N. San Juan6, F. Rascón7, C. Serna-Candel8, J. Matias-Guiu9, J.A. Barcia10 Laboratory Regenerative Neurosciences. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN1, Neurology Department. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN2, Laboratory Regenerative Neurosciences. Institute of Neuro-sciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN3, Laboratory Regenerative Neurosci-ences. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN4, Laboratory Regenerative Neurosciences. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Ma-drid, SPAIN5, Laboratory Regenerative Neurosciences. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN6, Neurosurgery Department. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN7, Neurology Department. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN8, Neurology Department. Institute of Neuro-sciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN9, Neurosurgery Department. Institute of Neurosciences, IdISSC. Hospital Clinico San Carlos, Madrid, SPAIN10 Stroke represents an attractive target for stem cell therapy. Although different types of cells have been employed in animal models with variable results, the use of human adipose derived stem cells (h-ADSc) has demonstrated favourable characteristics in the treatment of diseases or lesions with in-flammatory substrate, but experience in their intracerebral administration is lacking. The purpose of this study is to evaluate the effect and safety of the intracerebral application of h-ADSc in Athymic Nude mouse with stroke (chemical thrombosis of the ACM - Cl3Fe Model) and without stroke. A first group of Athymic Nuede mouse were injected with ADSc at a concentration of 4x104 / μL un-der deep anaesthesia and stereotactic procedures, a second group received the same cell concentra-tion after a stroke, and a third group only stroke and no cells. After 7 or 21 days, the animals were performed PET (FDG) and NMR; subsequently, were sacrificed for histological evaluation (HuNu, GFAP, IBA-1, Ki67, DCX) of the penumbra area and ipsilateral subventricular zone (i-SVZ). None of the animals implanted with cells, seizures or neurologic deficits were observed. PET results and NMR showed no abnormalities in animals implanted cells. We did not find proliferating h-ADSc cells or tumour formation. However, an increase of capillaries and modulation of astrocyte and mi-croglial activity, increased proliferation in the i-SVZ and increased number of neuroblasts in the area of injury were found. These results suggest that h-ADSc modulate inflammation, promote en-dogenous neurogenesis, while they do not proliferate or migrate in the brain. These data confirm and suggest the safety of cell therapy with h-ADSc. Fig 2 NeuN staining in perinfarct and infarct area of different groups. (Bar=50μm ) 1) Red represents Neuron and blue for nuclear. 2) A1, A2, A3 and A4 are NeuN staining of normal temperature group, 6 hours delayed mild hypo-thermia group, 2 hours delayed mild hypothermia group, and sham group, respectively, in perinfarct area. B1, B2, B3, B4 stands for NeuN staining of normal temperature group, 6 hours delayed mild hypothermia group, 2 hours delayed mild hypothermia group, and sham group, respectively, in in-farct area.
Karger_ESC London_2013
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