22. European Stroke Conference 934 Interventional neurology miRNA expression profiles in cerebrospinal fluid from patients with acute ischemic stroke ver-sus other neurological diseases T. Christensen1, A.M. Strømmen2, A.-B. Nygaard Hillig3, K. Jensen4 Department of Neurology, Copenhagen University Hospital Hilleroed, Hilleroed, DENMARK1, Department of Neurology, Copenhagen University Hospital Hilleroed, Hilleroed, DENMARK2, Department of Clinical Biochemistry, Copenhagen University Hospital Hilleroed, Hilleroed, DEN-MARK3, Department of Neurology, Copenhagen University Hospital Hilleroed, Hilleroed, DEN-MARK4 Background Emerging evidence suggests that miRNA expression profiling of cerebrospinal fluid (CSF) may have potential as a diagnostic biomarker with the ability to distinguish between different neurological dis-eases. 840 © 2013 S. Karger AG, Basel Scientific Programme The purpose of our study was two-fold: 1) To determine whether miRNAs can be detected in CSF from patients with ischemic stroke, and 2) to compare the miRNA expression profiles in CSF from ischemic stroke patients with CSF from patients with other neurological diseases in order to address whether miRNA expression profiling of CSF has potential usefulness as a diagnostic biomarker in ischemic stroke. Methods CSF from patients with ischemic stroke admitted to the acute stroke unit at Hilleroed Hospital (n = 10) and patients going through diagnostic workup for other neurological diseases (control group, n = 10) was collected by lumbar puncture. miRNA expression profiles in the CSF were analyzed by a commercially available microarray analysis technique (miRCURY LNA™ microRNA Array, Ex-iqon A/S, Denmark). Results The preliminary results of the analyses demonstrate that a number of different miRNAs are detect-able in CSF from patients with both ischemic stroke and other neurological diseases. Final sample analysis is currently being completed and additional detailed data addressing whether the miRNA profiles in CSF from patients with ischemic stroke are significantly different from CSF miRNA pro-files from patients with other neurological diseases will be presented at the conference. Conclusion The study demonstrates that it is possible to detect and profile miRNA in CSF from patients with ischemic stroke expression profiles and the study hence adds to our scant knowledge regarding miR-NA expression in CSF from patients with neurological disease. It is speculated that miRNA expres-sion profiling in CSF from patients with ischemic stroke might aid establishing the etiology of the stroke. 935 Interventional neurology External Carotid Artery (ECA) Angioplasty and Stenting of a recurrent stroke with hemody-namically significant ECA stenosis revealed by carotid duplex sonography Y.S. Ha1, S.S. Park2, J.S. Cheong3, H.S. Lee4, H.Y. Park5, H. Chang6, K.H. Cho7, K.O. Lee8 Department of Neurology, College of Medicine, Wonkwang University, Iksan, SOUTH KO-REA1, Department of Neurology, College of Medicine, Wonkwang University, Iksan, SOUTH KOREA2, Department of Neurology, College of Medicine, Wonkwang University, Iksan, SOUTH KOREA3, Department of Neurology, College of Medicine, Wonkwang University, , 4, Department of Neurology, College of Medicine, Wonkwang University, , 5, Department of Neurology, College of Medicine, Wonkwang University, , 6, Department of Neurology, College of Medicine, Wonkwang University, Iksan, 7, Department of neurology, College of Medicine, Konyang University, Daejeon, SOUTH KOREA8 Neurologic symptoms in the setting of internal carotid artery (ICA)occlusion may be due to embolic events through the external carotid artery (ECA) circulation, hemodynamic insufficiency resulting from inadequate collateral development. ECA endovascular intervention has been used to prevention neurologic events from the two mechanisms. Particularly, the management of ipsilateral ECA steno-sis is important when collateral flow of the Circle of Willis is impaired. We report a case of angio-plasty and stenting for ECA with angiographic mild stenosis but physiologically severe stenosis. The authors performed ECA angioplasty and stenting in a 87-year-old man who had presented with recurrent right hemispheric ischemic symptoms stemming from right proximal ECA stenosis and with right ICA occlusion. The patient had angiographic ECA stenosis of less than fifty percent but functionally more severe stenosis by carotid duplex sonography. Perfusion MRI was severe perfu-sion delay in the majority of the right MCA territory. The authors decided performed ECA angio-plasty and stenting to augment cerebral perfusion. He immediately improved his symptoms. Post-in-tervention perfusion MRI demonstrated to improve hypoperfusion. In our case, the application of ECA angioplasty and stenting augmented cerebral perfusion and im-proved clinical symptoms. Carotid duplex sonography provides physiologic information relative to blood flow velocity that is complimentary to the anatomic data provided by angiography for assess-ing the potential for hemodynamic significance of external carotid stenosis. Even though mild steno-sis of ECA on angiographic finding, ECA angioplasty and stenting can increase cerebral perfusion in the setting of hemodynamically significant ECA stenosis with ipsilateral ICA occlusion and poor collateral flow of the Circle of Willis.
Karger_ESC London_2013
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