22. European Stroke Conference 867 Intracerebral/subarachnoid haemorrhage and venous diseases DIFFERENTIAL MODULATION OF THE CELLULAR IMMUNE SYSTEM IN SPONTA-NEOUS INTRACEREBRAL HEMORRHAGE S. Illanes1, F. Vial2, V. Simon3, J. Gajardo4, V. Díaz5, P. Congett6 Instituto de Ciencias, Universidad del Desarrollo-Clínica Alemana, Santiago, CHILE1, Instituto de Ciencias, Universidad del Desarrollo-Clínica Alemana, Santiago, CHILE2, Instituto de Ciencias, Universidad del Desarrollo-Clínica Alemana, Santiago, CHILE3, Clínica Dávila. Universidad de los Andes, Santiago, CHILE4, Hospital Clínico Universidad de Chile, Santiago, CHILE5, Instituto de Ciencias, Universidad del Desarrollo-Clínica Alemana, Santiago, CHILE6 Background and Purpose: Intracerebral hemorrhage (ICH) accounts for 15-25% of acute stroke, and is associated with high mortality and morbidity rates. Experimental studies have identified a number of immunological fac-tors that are involved in the pathophysiology of ICH, including leukocyte brain infiltration, initia-tion of the complement cascade and microglial activation. Our objective was to analyze periph-eral immune cell response and lymphocytes subpopulation in blood in a prospective cohort of ICH patients. Methods: Differential blood leukocyte counting was performed and lymphocyte subpopulations were further characterized by flow cytometry in blood 24h, 3d and 7d after the ICH presentation. We compared absolute numbers of helper T cells (CD3+CD4+), cytotoxic T cells (CD3+CD8+), B cells (CD19+), regulatory T cells (CD4+CD25+Foxp3+), Natural Killer cells (CD3+CD16+CD56+) and the per-centage of regulatory T cells within the CD4+ population in blood in patients with large hematoma volume (>25ml) versus small hemorrhage size (<25ml) and presence of intraventricular bleeding versus patients without blood in cerebral ventricles. Results: 16 patients completed the study. Patients with large hematoma volume had a bigger leucocytes num-ber 24h, 3d and 7d after ICH presentation compared with small hematoma volume patients (ANO-VA p<0.04) with a significant increase regulatory T cells number (ANOVA p=0.03) and a bigger proportion of infections during the first seven days of follow up (X2 p<0.01). Patient with ICH + in-traventricular bleeding had a smaller leucocyte number count 24h, 3d and 7d after ICH presentation compared with patients without blood in the cerebral ventricles (ANOVA p<0.001) and a significant increase regulatory T cells number (ANOVA p=0.03) Conclusion: Hemorrhage size is a major determinant of post-stroke systemic immune modulation while the pres-ence of intracerebral bleeding determines a profound immunosuppresion. Hematoma size associates 804 © 2013 S. Karger AG, Basel Scientific Programme with more infections.
Karger_ESC London_2013
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