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22. European Stroke Conference 505a Translational stroke research Temporal differentiation of inflammatory cells in rodent brain infarcts F.F. Johansen1, N.V. Grønberg2, K.R. Jacobsen3, H. Hasseldam4, U. Kristiansen5 Copenhagen Experimental Stroke Unit, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DENMARK1, University of Copenhagen, Copenhagen, DENMARK2, University of Copenhagen, Copenhagen, DENMARK3, University of Copenhagen, Copenhagen, DENMARK4, University of Copenhagen, Copenhagen, DENMARK5 Background: The immune system is a possible target for delayed stroke treatment. We investigated the temporal differentation of inflammatory cells in rat and mice brain infarcts to identify a possible therapeutic strategy. We performed both a literature study, and experimental studies in mice surviv-ing permanent middle cerebral artery occlusion (pMCAO). Additionally, the influence of the antiin-flammatory drug Meloxicam on infarct volume was evaluated. Methods: A meta-analysis of 32 criteria-selected articles with pMCAO or transient MCAO in adult, male mice or rats were included. The numerical highest estimation in each article was set at 100 % and other values calculated in % of this to characterise the time course. Experimental data were ob-tained from 3x5 adult male mice, surviving 1, 3 or 8 days after pMCAO. One additional group of mice (n=8) received Meloxicam just before pMCAO and survived 8 days.Brain sections were im-munnostained with anti-CD3 and cells counted systematically. Results: Meta-analysis demonstrated that neutrophils peak between 24-72 h and macrophage/mi-croglia peak later than 72 h after stroke. Statistics (ANOVA) show that occlusion time significant-ly influences the time course of neutrophil (p < 0,001) and macrophage/microglia infiltration (p = 0,004): long occlusion time (120 min vs 60/30 min) increased early leucocyte infiltration, whereas this surprisingly was inverse for macrophages/microglial infiltration. Our own experiments showed that T-cells inceased steadily until day 8 (Spearmann Rank Order Correlation; p = 0,0162): the number of T-cells increased in the penumbra and decreased in the infarct core with time (ANOVA; p=0,005). Meloxicam treatment reduced the infarct size. Conclusion: Long occlusion time increase early leucocyte infiltration and decreased early macro-phages/ microglial infiltration. Total T-cells incease steadily such that the number increases in pen-umbra and decreases in the infarct core. Meloxicam reduces infarct size. 556 © 2013 S. Karger AG, Basel Scientific Programme


Karger_ESC London_2013
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