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22. European Stroke Conference 501 Translational stroke research Do patients who take part in stroke research differ from non-participants? Implications for generalizability of results. G. Silver1, L. Busija2, L.W. Tao3, D. Liew4, L. Weir5, B. Yan6, S. Davis7, P.J. Hand8 Royal Melbourne Hospital, Melbourne, AUSTRALIA1, Royal Melbourne Hospital, Melbourne, AUSTRALIA2, Royal Melbourne Hospital, Melbourne, AUSTRALIA3, University of Melbourne, Melbourne, AUSTRALIA4, Royal Melbourne Hospital, Melbourne, AUSTRALIA5, Royal Mel-bourne Hospital, Melbourne, AUSTRALIA6, Royal melbourne Hospital, Melbourne, AUSTRALIA7, Royal Melbourne Hospi8 Background and purpose. Clinical research is vital for expanding knowledge of treatment effective-ness among stroke patients, providing that results of research can be generalised to the broader pa-tient population. The aims of this study were to determine the prevalence of participation in clinical research studies by stroke patients and to compare demographic and clinical profiles of participants and non-participants in stroke research. Methods. 5,235 consecutive stroke or TIA patients admitted to the Stroke Care Unit (SCU) of the Royal Melbourne Hospital (RMH) between January 2004 and December 2011 were studied. As-sociations between research participation and patient characteristics were initially assessed using chi-square or Mann-Whitney tests, followed by a multivariable logistic regression model conducted using generalised estimating equations approach, to account for multiple admissions. Results. Five hundred and fifty eight SCU patients (10.7%) took part in at least one of the 33 clin-ical research studies during the study period. Transfer from another hospital (OR=0.35, 95%CI 0.22-0.55), worse premorbid function (OR=0.61, 95%CI 0.54-0.70), being single (OR=0.61, 95%CI 0.44-0.84) or widowed (OR=0.77, 95%CI 0.60-0.99), non-English language (OR=0.67, 95% CI 0.53-0.85), high socioeconomic status (OR=0.74, 95%CI 0.59-0.93), residence outside Melbourne (OR=0.75, 95%CI 0.60-0.95), weekend admission (OR=0.78, 95%CI 0.64-0.94), and a history of atrial fibrillation (OR=0.79, 95% CI 0.63-0.99) were associated with lower odds of research partici-pation. 554 © 2013 S. Karger AG, Basel Scientific Programme Conclusions. This study provides insights into clinical, demographic, and socio-economic factors as-sociated with participation in clinical stroke research and identifies factors that could potentially be targeted to enhance generalizability of stroke research studies. 502 Translational stroke research HETEROGENEITY OF ACUTE ADC MAPS PREDICTS TISSUE PRESERVATION: HY-DROGEN SULPHIDE EXPOSURE AFTER ACUTE ISCHEMIA IN A RAT MODEL I. Henriques1, M. Gutiérrez-Fernández2, B. Rodríguez-Frutos3, J. Ramos4, T. Navarro5, S. Cerdan6, J. Ferro7, E. Díez-Tejedor8 Neurosciences and Cerebrovascular Research Lab IdiPAZ, Champalimaud NC Program, Cen-tro Hospitalar LZC, Faculdade de Medicina Universidade de Lisboa, Lisboa, PORTUGAL1, Neurosciences and Cerebrovascular Research Lab IdiPAZ, Neurology Department Hospital Uni-versitario la Paz, Madrid, SPAIN2, Neurosciences and Cerebrovascular Research Lab IdiPAZ, Neu-rology Department Hospital Universitario la Paz, Madrid, SPAIN3, Neurosciences and Cerebrovas-cular Research Lab IdiPAZ, Neurology Department Hospital Universitario la Paz, Madrid, SPAIN4, SIERMAC Universidad Autónoma de Madrid, Madrid, SPAIN5, SIERMAC Universidad Autónoma de Madrid, Madrid, SPAIN6, Faculdade de Medicina da Universidade de Lisboa, Lisboa, PORTU-GAL7, Neurosciences and Cerebrovascular Research Lab IdiPAZ, Neurology Department Hospital Universitario la Paz, Madrid, SPAIN8 Background: Hydrogen sulphide (H2S) modulates antinecrotic and antiapoptotic mechanisms in the context of acute ischemia. We studied the effect of H2S exposure after acute ischemic stroke in a rat MCA occlusion model and looked for acute image (MRI) prognostic factors of very early brain tis-sue preservation. Methods: Young adult male Sprague-Dawley rats were distributed into 3 groups: 1-Control: Sur-gery + permanent MCAO; 2 – Treated: Surgery + permanent MCAO + inhalation of 40 ppM H2S; 3- Sham. We analyzed functional outcome (Roger modified scale) and lesion volume (Hematoxiline/ Eosine and T2, DWI and PWI MRI) at 24h and day 14. We also analyzed GFAP expression, cell death by TUNEL at border zone infarct, and protection NOX-4 enzyme by immunohistochemistry labeling and Western Blot (W/B). Results: We observed a decrease in infarct size in treated animals at day 14 in T2 MRI (*p= 0, 0383) and in H/E (*p= 0.0266). At 24 h ADC maps showed no significant difference in infarct size (p= 0, 7613), but functional outcome was better both at 24h (**p = 0, 0044) and day 14 (***p = 0, 0009). Control animals showed, at 24h, homogeneous ADC maps and treated ones heterogeneity; this ob-servation correlated in intralesional PWI, to areas of higher CBF (**p= 0,0056). There was signifi-cant less expression of TUNEL at the border zone infarct (*p= 0, 0307) and less GFAP expression in treated animals (p<0.05). Conclusion: Exposure to H2S after acute MCA occlusion led to improved functional recovery de-spite no difference in MRI lesion volume at 24h. This discrepancy between size and function at 24h could be partially explained by a better tissue structure preservation in treated animals because im-age heterogeneity in ADC maps corresponds to areas of higher CBF. This is also supported by GFAP downregulation and less apoptotic expression in treated animals. In our MCAO rat model, ADC maps intralesion heterogeneity at 24 h predicted better functional outcome.


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