22. European Stroke Conference 33 Acute stroke: new treatment concepts Human serum microRNA profiles after ischaemic stroke C.R. Breen1, C. Groome2, J.D. McClure3, I. M. Macrae4, A. H. Baker5, J. Dawson6, L.M. Work7 University of Glasgow, Glasgow, UNITED KINGDOM1, University of Glasgow, Glasgow, UNIT-ED KINGDOM2, University of Glasgow, Glasgow, UNITED KINGDOM3, University of Glasgow, Glasgow, UNITED KINGDOM4, University of Glasgow, Glasgow, UNITED KINGDOM5, Uni-versity of Glasgow, Glasgow, UNITED KINGDOM6, University of Glasgow, Glasgow, UNITED KINGDOM7 Background Recent pre-clinical1,2 & clinical3 studies suggest micro RNAs (miRNAs) may have a role in de-velopment of (and recovery after) stroke. Identification of a miRNA signature specific to stroke could serve to facilitate diagnosis and also provide novel therapeutic targets to improve outcomes. We screened serum samples for candidate miRNAs in a cohort of patients with ischaemic stroke and compared them to a cohort of patients with a stroke mimic. Methods Serum samples were obtained from patients presenting with suspected ischaemic stroke at 48 hours following symptom onset. . All patients were assessed by a specialist stroke service and diagnoses were adjudicated in a weekly meeting. A diagnosis of stroke (cases, n=15) was assigned where brain imaging showed an ischaemic lesion in a territory relevant to symptoms. A diagnosis of non-stroke (controls, n=15) was made where brain imaging did not and a firm alternative diagnosis was made. Controls were age and gender matched. Total RNA was extracted and a miRNA array performed using the Openarray platform (758 miRNA represented). Data were analysed using data-assist soft-ware &statistical comparisons made between groups where miRNA expression was measured in >70% of at least one sample group. Results Of 758 miRNAs, 538 were detected in the serum samples. Comparison of the raw cycle threshold (Ct) values of cases and controls identified 11 miRNAs which were significantly up-regulated in the stroke population (p<0.05) (Table 1). Conclusion miRNA are present and detectable early after ischaemic stroke. Further, several miRNAs are differ-entially expressed in cases of stroke compared to age and gender matched controls presenting with mimic. Further study is needed to validate findings in a larger cohort and to identify the pathophysi-ological significance of individual miRNAs. References 1. Dharap A et al. JCBFM. 2009;29:675–87. 2. Jeyaseelan K et al. Stroke. 2008;39:959–66. 3. Tan KS et al. PLoS One. 2009;4(11):e7689. 294 © 2013 S. Karger AG, Basel Scientific Programme Ct Value miRNA Non-stroke Stroke 27a 25.8 (22-31) 23.6 (20-25) 374 26.7 (22.5-28.5) 23.6(20-27) 331 25 (21-30) 22.5 (19.5-26.5) 590-5p 25 (23-28) 24 (22-25) 885-5p 26 (24-30) 24 (11.8-26) 93 25 (23-28) 24 (20-27) 25 24 (21-26) 21 (18-26) 19b 20 (16-22) 17 (14-23) 24 21 (17-23) 18.5 (14-24) 451 19 (16-24) 17 (14-21) 21 23 (20-27) 21 (19-24) Table 1 –miRNAs found to be significantly increased following stroke. Values represented as medi-an Ct value with the Ct range in brackets.
Karger_ESC London_2013
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